Gene technology

Gene technology

Academic year 2018/2019, spring semester

Type of course and credits given: elective „B”, 2 credits

Time: Monday, 16:15-18:00

Venue: „Kemény Armand” lecture hall (Department of Physiology and Biochemistry)

Exam: written test

 

Topics and schedules of the lectures:

Date Topic Lecturer
18 February Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. Dr. Mátis Gábor
25 February The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. Dr. Mátis Gábor
4 March Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. Dr. Orbán Kata
11 March Examination methods in the molecular biology I.: PCR, nucleotide sequencing. Dr. Harrach Balázs
18 March Examination methods in the molecular biology II.: qRT-PCR, Western blot. Dr. Mackei Máté
25 March Cell cultures and their application in the molecular biology. Dr. Orbán Kata
1 April Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. Dr. Mátis Gábor
8 April Written exam

 


Further information:
Matis.Gabor@univet.hu